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IMOTO LAB
 

Dissecting the synaptic membrane fission and the trafficking system at milliseconds and nanometer resolution

Publications

<2024>

33. Dynamin 1xA interacts with Endophilin via its extended C-terminus for ultrafast endocytosis.       
       
Imoto, Yǂ., Xue, Jǂ., Raychaudhuri, S., Itoh, K., Ma, Y., Luo, L., Hains, P., Annie, Q., Novellel, A.E., Flores-Rodriguez, N., Ha, T., Watanbe, S., & Robinson, PJ.       
       EMBO J (2024), doi.org/10.1038/s44318-024-00145-x
       bioRxiv (2023), doi: 10.1101/2023.09.21.558797       
       ǂ co-first authors
       Highlighted in EMBO J.  (2024) 43:3309-3311, doi/10.1038/s44318-024-00179-1.     
       Cover art in EMBO J (2024) 43(16), https://www.embopress.org/toc/14602075/current


<2023>

32. Membrane compression by synaptic vesicle exocytosis triggers ultrafast endocytosis.
       Jing, Hǂ., Ogunmowo, Tǂ., Raychaudhuri, Sǂ., Kusick, G.Fǂ., Imoto, Y., Li, S., Itoh, K., Chapman, E.R., Ha, T., Watanabe, S. and Liu, J.
       Nat. Commun. (2023), 14 (1): 288. doi: 10.1038/s41467-023-38595-2
       ǂ co-first authors

< 2022 >
 
31. Dynamin is primed at endocytic sites for ultrafast endocytosis.
       Imoto, Y*., Raychaudhuri, S., Ma, Y., Fenske, P., Sandoval, E., Itoh, K., Blumrich, EM., Matsubayashi, T., Mamer, L., Zarebidaki, F., Söhl-Kielczynski, 
       B., Trimbuch, T., Nayak, S., Iwasa, J.H., Liu, J., Wu, B., Ha, T., Inoue, T., Jorgensen, E.M., Cousin, M.A., Rosenmund, C*., & Watanabe, S*.
       Neuron (2022), 110(17): 2815-2835. e13. doi: 10.1016/j.neuron.2022.06.010
       *co-corresponding authors.
       Highlighted in Nature review neuroscience (2022) 23(9):520-521, doi:10.1038/s41583-022-00626-0.
       Highlighted in Neuron (2022) 110(17):2705-2707, doi:10.1016/j.neuron.2022.08.002.
 
30. Axonal transport of Hrs is activity dependent and facilitates synaptic vesicle protein degradation.
       Birdsall, V., Kirwan, K., Zhu, M., Imoto, Y., Wilson, S. M., Watanabe, S., & Waites, C. L.
       Life Science Alliance (2022), 5(10). doi: 10.26508/lsa.202000745
 
29. Dynamin forms liquid-like condensates at synapses to support ultrafast endocytosis.
       Imoto, Y., Ma, Y., Itoh, K., Blumrich, E.M., Matsubayashi, H.T., Liu, J., Wu, B., Cousin, M.A., Ha, T., Inoue, T. and Watanabe, S.
       bioRxiv (2022). doi: 10.1101/2022.06.01.494432

< 2021 >
 
28. Smooth Loop-Like Mitochondrial Nucleus in the Primitive Red Alga Cyanidioschyzon merolae Revealed by Drying Treatment.
       Kuroiwa, T., Yagisawa, F., Fujiwara, T., Misumi, O., Nagata, N., Imoto, Y., Yoshida, Y., Mogi, Y., Miyagishima, S.Y. and Kuroiwa, H.
       Cytologia (2021), 86: 89-96. doi: 10.1508/cytologia.86.89
 
<2020>
 
27. Molecular Basis of Mitochondrial and Peroxisomal Division Machineries.
       Imoto, Y*., Itoh, K., & Fujiki, Y*.
       Int. J. Mol. Sci. (2020), 21(15): 5452. doi: 10.3390/ijms21155452
       *co-corresponding authors.

26. Mammalian homologue NME3 of DYNAMO1 regulates peroxisome division.
       Honsho, M., Abe, Y., Imoto, Y., Chang, ZF., Mandel, H., Falik-Zaccai, TC., & Fujiki, Y.
       Int. J. Mol. Sci. (2020), 21: 8040. doi: 10.3390/ijms21218040

25. ESCRT machinery mediates cytokinetic abscission in the unicellular red alga Cyanidioschyzon merolae.
       Yagisawa, F., Fujiwara, F., Takemura, T., Kobayashi, F., Sumiya, N., Miyagishima, S., Nakamura, S., Imoto, Y., Misumi, O., Tanaka, K., Kuroiwa, H., & 
       Kuroiwa, T.
       Front. Cell Dev. Biol. (2020), 8: 169. doi: 10.3389/fcell.2020.00169

24. Evolutionary significance of the ring-like plastid nucleus in the primitive red alga Cyanidioschyzon merolae as revealed by drying.
       Kuroiwa, T., Ohnuma, M., Imoto, Y., Yagisawa, F., Misumi, O., Nagata, N., & Kuroiwa, H.
       Protoplasma (2020), 1-10.  doi: 10.1007/s00709-020-01496-y

23. Mitotic karyotype of the primitive red alga Cyanidioschyzon merolae 10D.
       Kuroiwa, T., Yagisawa, F., Fujiwara, T., Inui, Y., Matsunaga, T.M., Katoi, S., Matsunaga, S., Nagata, N., Imoto, Y., & Kuroiwa, H.
       Cytologia (2020), 85(2): 107-113. doi: 10.1508/cytologia.85.107

22. Recent insights into peroxisome biogenesis and associated diseases.
       Fujiki, Y., Abe, Y., Imoto, Y., Tanaka, A.J., Okumoto, K., Honsho, M., Tamura, S., Miyata, N., Yamashita, T., Chung, W.K., & Kuroiwa, T. 
       J. Cell Sci. (2020), 133(9). doi: 10.1242/jcs.236943

 
<2019>
 
21. Dynamics of nucleoside diphosphate kinase protein DYNAMO2 correlates with global GTP level during cell cycle of Cyanidioschyzon merolae.
       Imoto, Y., Abe, Y., Okumoto, K., Ohnuma, M., Kuroiwa, H., Kuroiwa, T., & Fujiki, Y*.
       Proc. Japan Acad. Ser. B (2019), 95(2): 75-85.  doi: 10.2183/pjab.95.007

20. Cyanidioschyzon merolae aurora kinase phosphorylates evolutionarily conserved sites on its target to regulate mitochondrial division.
       Kato, S., Okamura, E., Matsunaga, T. M., Nakayama, M., Kawanishi, Y., Ichinose, T., Iwane, A. H., Sakamoto, T., Imoto, Y., Ohnuma, M., Nomura, Y.,         Nakagami, H., Kuroiwa, H., Kuroiwa, T., & Matsunaga, S.
       Commun. Biol. (2019), 2(1): 477. doi: 10.1038/s42003-019-0714-x
 
< 2018 >
 
19. Onsite GTP fuelling via DYNAMO1 drives division of mitochondria and peroxisomes.
       Imoto, Y., Abe, Y., Honsho, M., Okumoto, K., Ohnuma, M., Kuroiwa, H., Kuroiwa, T., & Fujiki, Y.
       Nat. Commun. (2018), 9(1): 4634. doi: 10.1038/s41467-018-07009-z
 
< 2017 >
 
18. Defining dynaminbased ring organizing center on the peroxisome-dividing machinery isolated from Cyanidioschyzon merolae.
       Imoto, Y., Abe, Y., Okumoto, K., Honsho, M., Kuroiwa, H., Kuroiwa, T., & Fujiki Y.
       J. Cell Sci. (2017), 130(5): 853-867. doi: 10.1242/jcs.199182
      Highlighted in J. Cell Sci (2017) A ring organising centre during organelle division, 130 (5): e0502.

17. Glycosyltransferase MDR1 assembles a dividing ring for mitochondrial proliferation comprising polyglucan nanofilaments.
       Yoshida, Y., Kuroiwa, H., Shimada, T., Yoshida, M., Ohnuma, M., Fujiwara, T., Imoto, Y., Yagisawa, F., Nishida, K., Hirooka, S., Misumi, O., Mogi, Y.,             Akakabe, Y., Matsushita, K., & Kuroiwa, T.
       Proc. Natl. Acad. Sci. USA (2017), 114 (50): 13284-13289, doi: 10.1073/pnas.1715008114
 
< 2016 >
 
16. Genome size of the ultrasmall unicellular freshwater green alga, Medakamo hakoo 311, as determined by staining with 4′, 6-diamidino-2
       -phenylindole after microwave oven treatments: II. Comparison with Cyanidioschyzon merolae, Saccharomyces cerevisiae (n, 2n), and Chlorella
       variabilis
.
       Kuroiwa, T., Ohnuma, M., Imoto, Y., Misumi, O., Nagata, N., Miyakawa, I., Fujishima, M., Yagisawa, F., & Kuroiwa, H.
       Cytologia (2016), 81(1): 69-76. doi:10.1508/cytologia.81.69
 
< 2015 >
 
15. Cytological Evidence of Cell Nuclear Genome Size of a New Ultra-Small Unicellular Freshwater Green Alga, “Medakamo hakoo” strain M-
       hakoo 311. I. Comparison with Cyanidioschyzon merolae and Ostreococcus tauri.
       Kuroiwa, T., Ohnuma, M., Nozaki, H., Imoto Y., Misumi, O., & Kuroiwa, H.  doi:10.1508/cytologia.80.143
       Cytologia (2015), 80(2): 143-150
 
< 2014 >
 
14. Lipid droplet formation in cells of the filamentous green alga Klebsormidium nitens as revealed by BODIOY-DiOC6 and BODIPY-nile red double-
       staining microscopy.
       Kuroiwa, T., Ohnuma, M., Imoto, Y., & Kuroiwa, H.
       Cytologia (2014), 79(4): 501-507.  doi:10.1508/cytologia.79.501
 
< 2013 >
 
13. The kinesin-like protein TOP promotes Aurora localisation and induces mitochondrial, chloroplast and nuclear division.
       Yoshida, Y., Fujiwara, T., Imoto, Y., Yoshida, M., Ohnuma, M., Hirooka, S., Misumi, O., Kuroiwa, H., Kato, S., Matsunaga, S., & Kuroiwa, T.
       J Cell Sci. (2013), 126(11): 2392-2400. doi: 10.1242/jcs.116798

12. Single-membrane-bounded peroxisome division revealed by isolation of dynamin-based machinery. 
       Imoto Y., Kuroiwa H., Yoshida Y., Ohnuma M., Fujiwara T., Yoshida M., Nishida K., Yagisawa F., Hirooka S., Miyagishima SY., Misumi O., Kawano S.,
       & Kuroiwa, T. (2013),  Proc. Natl. Acad. Sci. USA, 110 (23): 9583-9588. doi: 10.1073/pnas.1303483110

11. Golgi  inheritance in the primitive red alga, Cyanidioschyzon merolae.
    Yagisawa F, Fujiwara T, Ohnuma M, Nishida K, Imoto Y., Yoshida Y, Kuroiwa H., & Kuroiwa T.
    Protoplasma (2013), 250(4): 943-948. doi: 10.1007/s00709-012-0467-6
 
< 2012 >
 
10. Identification of Peroxisome-Dividing Ring in Cyanidioschyzon merolae Based on Organelle Partner Hypothesis.
       Imoto Y., Kuroiwa, H., Ohnuma, M., Kawano, S., & Kuroiwa, T. 
       Cytologia (2012), 77(4): 1-8. doi: 10.1508/cytologia.77.515

9. Lipid Droplets of Bacteria, Algae and Fungi and a Relationship between their Contents and Genome Sizes as Revealed by BODIPY and DAPI
    Staining.
    Kuroiwa, T., Ohnuma, M., Imoto, Y., Misumi, O., Fujiwara, T., Miyagishima, S., Sumiya, N., & Kuroiwa H.
    Cytologia (2012), 77(3): 289-299. doi:10.1508/CYTOLOGIA.77.289

8. Identification of the plastid division gene PDR1.
    Yoshida, Y., Kuroiwa, H., Misumi, O., Yoshida, M., Ohnuma, M., Fujiwara, T., Yagisawa, F., Hirooka, S., Imoto, Y., Matsushita, K., Kawano, S., &
    Kuroiwa, T.
    Plant Morph. (2012), 24: 81-88. ​doi: 10.5685/plmorphol.24.81

7. Nuclear-Encoded Plastid Sigma Factor SIG6 Exclusively Contributes to Chloroplast Differentiation in Plastid Differentiation of Arabidopsis
    thaliana
.
    Hirooka, S., Hanaoka, M., Enami, K., Kanazawa, T., Sone, T., Imoto, Y., Ando, A., Kuroiwa, H., Kuroiwa, T., & Tanaka, K.
    Cytologia (2012), 77(1): 73-82. doi: 10.1508/cytologia.77.73

6. Mitotic inheritance of endoplasmic reticulum in the primitive red alga Cyanidioschyzon merolae.
     Yagisawa, F., Fujiwara, T., Kuroiwa, H., Nishida, K., Imoto, Y., & Kuroiwa, T.
     Protoplasma (2012), 249(4): 1129-1135. doi: 10.1007/s00709-011-0359-1
 
< 2011 >
 
5. The cell cycle, including the mitotic cycle and organelle division cycles, as revealed by cytological observations.
    Imoto, Y., Yagisawa, F., Yoshida, Y., Kuroiwa, H., & Kuroiwa, T.
    J. Electro. Micro. (2011), 60(supp_1): 117-136. doi: 10.1093/jmicro/dfr034

4. Involvement of elongation factor-1α in cytokinesis without actomyosin contractile ring in the primitive red alga Cyanidioschyzon merolae.
    Imoto, Y., Nishida, K., Yagisawa, F., Yoshida, Y., Ohnuma, M., Yoshida, M., Fujiwara, T., Kuroiwa, H., Kawano, S., & Kuroiwa, T.
    Cytologia (2011), 76(4): 431-437. doi:10.1508/cytologia.76.431

3. The Aurora kinase of a red alga Cyanidioschyzon merolae is related to both mitochondrial division and mitotic spindle formation.
    Kato, S., Imoto, Y., Ohnuma, M., Matsunaga, MT., Kuroiwa, H., Kawano, S., Kuroiwa, T., & Matsunaga S.     
    Cytologia (2011), 76(4): 455-462. doi:10.1508/cytologia.76.455
 
< 2010 >
 
2. Division of cell nuclei, mitochondria, plastids, and microbodies mediated by mitotic spindle poles in the primitive red alga Cyanidioschyzon
    merolae
.
    Imoto, Y., Fujiwara, T., Yoshida, Y., Kuroiwa, H., Maruyama, S., & Kuroiwa, T. 
    Protoplasma (2010), 241: 63-74. doi: 10.1007/s00709-010-0107-y

1. Chloroplasts divide by contraction of a bundle of nanofilaments consisting of polyglucan.
     Yoshida, Y., Kuroiwa, H., Misumi, O., Yoshida, M., Ohnuma, M., Fujiwara, T., Yagisawa, F., Hirooka, S., Imoto, Y., Matsushita, K., Kawano, S., &
     Kuroiwa, T.  
     Science (2010), 329 (5994), 949-953. doi: 10.1126/science.1190791

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